Y15 (1,2,4,5-Benzenetetramine tetrahydrochlor) is a potent and specificsmall-molecule FAK scaffolding inhibitor that inhibits its autophosphorylation activity, blocks tumor growth.

Y15 inhibited viability in ?thyroid cancer cell lines in a dose-dependent manner[1].

Apoptosis assay was done with PE Annexin V Apoptosis Detection Kit (BD) to identify apoptotic nuclei.?One hundred millimeter plates were seeded with TPC1, K1, BCPAP and TT cells at a concentration of 1 × 10^6 cells in normal growth media.?After overnight incubation, plates were treated with one of several doses of Y15 or left untreated.?After another 24 h of incubation, cells were collected with trypsin, and following centrifugation, were washed with 1× PBS.?Again, cells were centrifuged, PBS was removed and cells were resuspended in binding buffer and filtered through BD mesh tubes.?Annexin V and 7-AAD were added to the solution, vortexed and incubated for 15 minutes in the dark.?Binding buffer was added and the cells were analyzed by flow cytometry within 1 hour.