Aprotinin (Traskolan) is a broad-spectrum serine protease (BPTI) inhibitor that inhibits the activity of a number of different esterases and proteases. Aprotinin is an antifibrinolytic agent used to minimize hemorrhage during complex surgical procedures.

Trypsinogen:2 μM(Kd), Kallikrein:0.8 nM(Kd), Trypsin:0.06 pM(Kd), Chymotrypsin:9.5 nM(Kd)

方法：SARS-CoV-2 感染的 Caco2 细胞用 Aprotinin (0-20 µM) 处理 48 h，检测细胞病变效应 (CPE)。
结果：Aprotinin 显示出抗病毒作用，对 SARS-CoV-2 感染的 Caco2 细胞 CPE 形成的 IC50 范围为 0.81 µM-1.03 µM。[1]
方法：VSMCs 细胞用 Aprotinin (1-100 µM) 预处理 1 h，用细胞因子 (10 ng/mL IL-1β+25 ng/ml TNF-α) 处理 12 h，通过 Western Blot 和 RT-PCR 检测靶点基因和蛋白表达水平。
结果：Aprotinin 以浓度依赖的方式与细胞因子刺激 HO-1 蛋白质水平和 mRNA 水平的显著增加。[2]

方法：为研究体内抗流感病毒活性，将 Aprotinin (2 mg/kg) 静脉注射给致死性 A/PR/8/34 (H1N1) 病毒感染的 C57BL/6 小鼠，每天两次，持续 5 天。
结果：单独使用 Aprotinin 不会导致小鼠体重减轻。PBS 处理的小鼠在感染后 8 天存活率为 0%。同时，用 Aprotinin 治疗的小鼠组显示 75% 的存活率。[3]

Substrates and kinases are diluted in 50?mM Tris/HCl (pH?7.5), 0.1% 2-mercaptoethanol, 0.1?mM EGTA and 10?mM magnesium acetate. Reactions are initiated with [γ-32P]ATP (2500 c.p.m./pmol) to a final concentration of 0.1?mM and terminated after 15?min at 30°C by the addition of SDS and EDTA (pH?7.0) to final concentrations of 1.0% (w/v) and 20?mM respectively. After heating for 5?min at 100°C and separation by SDS/PAGE, the phosphorylated proteins are detected by autoradiography.

Mouse G8-1 myoblasts are plated DMEM + 20% FBS (maintenance medium), in which they remain undifferentiated. When cells reach approximately 40-50% confluence, different protease inhibitors are added to the culture media and cells are incubated overnight. Cells are then switched to differentiation-promoting media (DMEM + 10% horse serum ± protease inhibitor) and incubated for 7 days. (Only for Reference)