Sulforaphane is a natural isothiocyanate that activates Nrf2 and inhibits AMPK-dependent signaling. Sulforaphane has antitumor and anti-inflammatory activities by increasing the transcription of tumor suppressor proteins and inhibiting histone deacetylase activity.

方法: 人结直肠癌细胞 HT29 用 Sulforaphane (15 µM) 处理 24-96 h，使用 MTT assay 检测细胞活力。
结果: 15 µM 的 Sulforaphane 能够抑制细胞生长并诱导细胞死亡。24 h 时细胞死亡率已经达到75%，96 h 时几乎全部死亡。[1]
方法: 胃癌细胞 BGC-823 和 MGC-803 用 Sulforaphane (5-10 µM) 处理 48 h，使用 Western Blot 方法检测靶点蛋白表达水平。
结果: 当胃癌细胞用 Sulforaphane 处理时，细胞周期调节蛋白如 p53 和 p21 的表达水平以剂量依赖性的方式增加，而 S 期相关蛋白 CDK2 的表达水平显著降低。[2]

方法: 为检测抗炎活性，将 Sulforaphane (50 mg/kg) 单次腹腔注射给 BALB/c 小鼠，三天后注射 LPS 诱导急性肺损伤 (ALI) 。
结果: Sulforaphane 显著降低了 LPS 诱导的急性肺损伤小鼠的 LDH 活性、肺的湿干比、血清 IL-6 和 TNF-α 水平以及 NF-κB 蛋白表达。Sulforaphane 通过 Nrf2/ARE 途径对 LPS 诱导的 ALI 发挥保护作用。[3]

HT29 cells are seeded at low density (5×10^4 cells/mL) in 35- or 120-mm diameter Primaria dishes in standard medium containing 5% FCS. One day after seeding, the medium is changed, and HT29 cells are treated with sulforaphane (0-30 μM). An equivalent amount of the solvent (ethanol) is added to control cells (0.2% final concentration). The drug effect on cellular viability is evaluated using the MTT assay [1].

At age 47, 48, 49, 50, and 51 days, each animal receives by gavage either 0.5 mL of Emulphor EL-620 alone or the specified doses (75, 100, or 150 μM daily) of sulforaphane or compound 2, 3, or 4 in 0.5 mL of Emulphor EL-620. On day 50, 3 hr after administration of the vehicle or protector, all rats also receive an intragastric instillation of 8.0 mg of DMBA dissolved in 1.0 mL of sesame oil. This dose of DMBA is selected to produce a substantial tumor incidence, but not one so high as to overwhelm a potential chemoprotective effect. The animals are examined once weekly for the appearance and location of palpable tumors. At age 202 days, i.e., 152 days after carcinogen administration, all animals are euthanized with ether and weighed. The tumors are separated from the fat and connective tissue by dissection weighed and fixed in buffered 10% formalin. All tumors are identified microscopically by examination of stained sections [3].