NG25 is a potent dual TAK1 and MAP4K2 inhibitor, with IC50s of 149 nM and 21.7 nM, respectively.

GSK:56.4 nM, SRC:113 nM, Raf-1:7590 nM, MAP4K2:21.7 nM, ZAK:698 nM, TAK1:149 nM, EphB2:672 nM, ZC1/HGK:3250 nM, Abl/Arg:75.2 nM, EphB4:999 nM, FER:82.3 nM, EphA2:773 nM, Lyn:12.9 nM

Breast cancer cells were seeded in 12-well plates at 2?×?10^3 cells per well, then incubated with Dox alone or Dox with NG25 at indicated concentrations after 72?h and then were cultured in fresh medium without drug.?Two weeks later, cells were fixed and stained with methanol/crystal violet for 10?min, and photographed.?Each experiment was performed in triplicates[1].