NG25 is a potent dual TAK1 and MAP4K2 inhibitor, with IC50s of 149 nM and 21.7 nM, respectively.

MAP4K2:21.7 nM, TAK1:149 nM

NG25能够增强体外乳腺癌细胞对Dox治疗的敏感性。这种组合有可能成为一种有效且可行的治疗方案，既能最大化Dox的疗效，同时又能最小化Dox在治疗乳腺癌时的副作用[1]。

Breast cancer cells were seeded in 12-well plates at 2?×?10^3 cells per well, then incubated with Dox alone or Dox with NG25 at indicated concentrations after 72?h and then were cultured in fresh medium without drug.?Two weeks later, cells were fixed and stained with methanol/crystal violet for 10?min, and photographed.?Each experiment was performed in triplicates[1].