Importazole is an inhibitor of the nuclear transport receptor importin-β.

Importazole 在体外有效抑制 Ran/importin-β 相互作用。它特异性阻断 importin-β介导的核入运输，在 Xenopus 卵提取物和培养细胞中均是如此，同时不干扰 transportin 介导的核入运输或 CRM1 介导的核出运输。在有丝分裂期间添加时，importazole 妨碍了 importin-β 货物 FRET 探针的释放，并引起了预期和新颖的纺锤体组装缺陷[1]。此外，通过阻断 NF-κB 信号通路，它还能抑制多发性骨髓瘤细胞的增殖并诱导其凋亡[2]。

For all import and export experiments, HEK 293 cells stably expressing NFAT-GFP are grown on glass coverslips to approximately 50% confluency prior to drug treatment. In all cases, importazole is used at 40 μM and leptomycin B is used at 10 ng/ml. For controls, DMSO is used at a concentration of 0.4%. Ionomycin is added at 1.25 μM. Importazole and leptomycin B treatments are all for 1 hour. In all experiments cells are fixed with 4% formaldehyde prior to fluorescence microscopy. DNA is visualized with 1 μg/ml Hoechst dye. For quantification, 100 cells from each condition are analyzed and the percentage that shows nuclear accumulation of NFAT-GFP calculated.(Only for Reference)