HSD1590 is a highly effective and potent inhibitor of ROCK1 and ROCK2, with IC50 values of 1.22 nM and 0.51 nM, respectively. It demonstrates exceptional binding affinity to ROCK, with Kd values below 2 nM. Moreover, HSD1590 showcases minimal cytotoxicity, making it an ideal compound for various applications.

ROCK1:1.22 nM (IC50), ROCK2:0.51 nM (IC50)

HSD1590 (0.5-1 μM; 24 hours) exhibits an impressive attenuation in migration[1]. HSD1590 (0.5-10 μM; 12-24 horus) shows that the excellent migration inhibition observed is not due to cell death, but inhibition of live cell migration[1]. Cell Viability Assay[1]Cell Line: MDA-MB-23 cells Concentration: 0.5-10 μM Incubation Time: 12-24 hours Result: Exhibited approximately 80% viability at 12 hours and yielded an overall viability of 63% at 24 h.