ASK1-IN-3 is a highly potent and selective inhibitor of ASK1 kinase, demonstrating an IC 50 of 33.8 nM. This compound also exhibits inhibitory effects on various cell cycle regulating kinases. ASK1-IN-3 showcases remarkable induction of apoptosis in HepG2 cancer cells and displays potent activity in arresting the cell cycle [1].

ASK1-IN-3 (compound 14l) (10, 20 and 50 μM; 48 hours) induces PARP cleavage in a dose dependent manner, which indicates the induction of HepG2 cells apoptosis [1]. ASK1-IN-3 (1-16 μM; 24 hours) significantly arrests cycle progression at G1 phase [1]. Apoptosis Analysis Cell Line: HepG2 [1] Concentration: 10, 20 and 50 μM Incubation Time: 48 hours Result: Induced PARP cleavage in a dose dependent manner. Cell Cycle Analysis Cell Line: HepG2 [1] Concentration: 1, 2, 4, 8, and 16 μM Incubation Time: 24 hours Result: Significantly arrested cycle progression at G1 phase.