Antitumor agent-53 is a potent compound that effectively inhibits tumor growth. It achieves this by inducing cell cycle arrest at the G2/M phase and promoting apoptosis in HGC-27 cells through the inhibition of the PI3K/AKT pathway. Furthermore, Antitumor agent-53 holds promise for further research in the treatment of gastrointestinal tumors [1].

Antitumor agent-53 (compound 6f) (0, 0.22, 0.67, 2, 6, 18 μM; 72 h) shows anti-proliferation activity with IC 50 s of 3.10, 0.37, 4.01, >18, 7.87, 9.11 μM for HGC-27, HT-29, HepG-2, A549, MCF7, GES-1 cells [1]. Antitumor agent-53 (0.15, 0.3, 0.6 μM) shows anti-proliferative activity in HGC-27 and HT-29 cells with a dose-dependent manner [1]. Antitumor agent-53 (100, 200 μM) shows a certain inhibitory activity against Topo I at 200 μM [1]. Antitumor agent-53 (0.1, 0.3, 0.9 μM; 24 h) induces cell cycle arrest at the G2/M phase in HGC-27, HT-29 cells [1]. Antitumor agent-53 (0.1, 0.3, 0.9, 2.7 μM; 24 h) induces the apoptosis of HGC-27 and HT-29 cells in a concentration-dependent manner [1]. Antitumor agent-53 (0.15, 0.3, 0.6 μM; 24 h) inhibits the migration and invasion of HGC-27 cells in a concentration-dependent manner [1]. Antitumor agent-53 (0.1, 0.3, 0.9 μM; 24 h) suppresses the PI3K/AKT pathway to induce the apoptosis of HGC-27 cells [1]. Cell Proliferation Assay [1] Cell Line: HGC-27, HT-29, HepG-2, A549, MCF7, GES-1 cells Concentration: 0, 0.22, 0.67, 2, 6, 18 μM Incubation Time: 72 h Result: Showed anti-proliferation activity with IC 50 s of 3.10, 0.37, 4.01, >18, 7.87, 9.11 μM for HGC-27, HT-29, HepG-2, A549, MCF7, GES-1 cells. Cell Cycle Analysis [1] Cell Line: HGC-27, HT-29 cells Concentration: 0.1, 0.3, 0.9 μM Incubation Time: 24 h Result: Cells were arrest at the G2/M phase. Apoptosis Analysis [1] Cell Line: HGC-27, HT-29 cells Concentration: 0.1, 0.3, 0.9, 2.7 μM Incubation Time: 24 h Result: Induced the apoptosis of HGC-27 and HT-29 cells in a concentration-dependent manner. Western Blot Analysis [1] Cell Line: HGC-27 cells Concentration: 0.1, 0.3, 0.9 μM Incubation Time: 24 h Result: Suppressed the PI3K/AKT pathway to induce the apoptosis of HGC-27 cells.