购物车
  • 全部删除
  • TargetMol
    您的购物车当前为空

MK-0752

Rating icon 很棒
产品编号 T2625Cas号 471905-41-6

MK0752 是一种高效的、特异性的、可穿过血脑屏障的、口服活性的 γ-分泌酶(γ-secretase)抑制剂,对人 SH-SY5Y 细胞 Aβ40 呈剂量依赖性降低(IC50=5 nM)。它能够降低体内新生的中枢神经系统 Aβ。

MK-0752

MK-0752

Rating icon 很棒
纯度: 99.55%
产品编号 T2625Cas号 471905-41-6

MK0752 是一种高效的、特异性的、可穿过血脑屏障的、口服活性的 γ-分泌酶(γ-secretase)抑制剂,对人 SH-SY5Y 细胞 Aβ40 呈剂量依赖性降低(IC50=5 nM)。它能够降低体内新生的中枢神经系统 Aβ。

规格价格库存数量
1 mg
¥ 565
5日内发货
5 mg
¥ 1,520
5日内发货
10 mg
¥ 2,260
5日内发货
25 mg
¥ 3,850
5日内发货
50 mg
¥ 5,470
5日内发货
100 mg
¥ 7,660
5日内发货
1 mL x 10 mM (in DMSO)
¥ 1,670
现货
大包装 & 定制
加入购物车
TargetMol 的所有产品仅用作科学研究或药证申报,不能被用于人体,我们不向个人提供产品和服务。请您遵守承诺用途,不得违反法律法规规定用于任何其他用途。
实验操作小课堂
常见问题解答
查看更多

"MK-0752"的相关化合物库

选择批次:
纯度:99.55%
联系我们获取更多批次信息

产品介绍

生物活性
产品描述
MK-0752, a γ-secretase inhibitor, reduces Aβ40 production(IC50=5 nM).
靶点活性
β-Amyloid:5 nM
体外活性
在豚鼠体内,口服 MK-0752(10 -30 mg/kg),在血浆,大脑和脑脊液中Aβ40以剂量依赖性减少.在猕猴中, MK-0752(240 mg/kg)能够减少大脑中Aβ生成的生成.
体内活性
在人SH-SY5Y细胞中(IC50=5 nM),MK-0752能够以剂量依赖性减少Aβ40。
激酶实验
Protein kinase assays : Protein kinase assays are either done in-house by ELISA-based assay methods (Kit, KDR, PDGFRα, and PDGFRβ) or by a radiometric method. In-house ELISA assays used poly(Glu:Tyr) as the substrate bound to the surface of 96-well assay plates; phosphorylation is then detected using an antiphosphotyrosine antibody conjugated to HRP. The bound antibody is then quantitated using ABTS as the peroxidase substrate by measuring the absorbance at 405/490 nm. All assays uses purified recombinant kinase catalytic domains that are either expressed in insect cells or in bacteria. The Kit and EGFR protein used for in-house assays are prepared internally; other enzymes are obtained. Recombinant Kit protein is expressed as an NH2-terminal glutathione S-transferase fusion protein in insect cells and is initially purified as a nonphosphorylated (nonactivated) enzyme with a relatively high Km for ATP (400 μM). In some assays, an activated (tyrosine phosphorylated) form of the enzyme is prepared by incubation with 1 mM ATP for 1 hour at 30 °C. The phosphorylated protein is then passed through a desalting column to remove the majority of the ATP and stored at ?80 °C in buffer containing 50% glycerol. The resultant preparation has a considerably higher specific activity and a lower Km for ATP (25 μM) than the initial nonphosphorylated preparation. The inhibition of Kit autophosphorylation by OSI-930 is assayed by incubation of the nonphosphorylated enzyme at 30 °C in the presence of 200 μM ATP and various concentrations of OSI-930. The reaction is stopped by removal of aliquots into SDS-PAGE sample buffer followed by heating to 100 °C for 5 minutes. The degree of phosphorylation of Kit is then determined by immunoblotting for both total Kit and phosphorylated Kit.
化学信息
分子量442.9
分子式C21H21ClF2O4S
CAS No.471905-41-6
SmilesOC(=O)CC[C@H]1CC[C@@](CC1)(c1cc(F)ccc1F)S(=O)(=O)c1ccc(Cl)cc1
密度1.361 g/cm3
储存&溶解度
存储Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
溶解度信息
H2O: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 89 mg/mL (200.95 mM), Sonication is recommended.
Ethanol: 45 mg/mL (101.6 mM), Sonication is recommended.
溶液配制表
Ethanol/DMSO
1mg5mg10mg50mg
1 mM2.2578 mL11.2892 mL22.5785 mL112.8923 mL
5 mM0.4516 mL2.2578 mL4.5157 mL22.5785 mL
10 mM0.2258 mL1.1289 mL2.2578 mL11.2892 mL
20 mM0.1129 mL0.5645 mL1.1289 mL5.6446 mL
50 mM0.0452 mL0.2258 mL0.4516 mL2.2578 mL
100 mM0.0226 mL0.1129 mL0.2258 mL1.1289 mL

SCI 文献

计算器

  • 摩尔浓度 计算器
  • 稀释 计算器
  • 配液 计算器
  • 分子量 计算器

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法:
TargetMol | Animal experiments比如您的给药剂量是 10 mg/kg ,每只动物体重 20 g ,给药体积 100 μLTargetMol | Animal experiments 一共给药动物 10 只 ,您使用的配方为 5% TargetMol | reagent DMSO+ 30%PEG300+ 5%Tween 80 + 60% ddH2O. 那么您的工作液浓度为 2 mg/mL
母液配置方法: 2 mg 药物溶于 50 μLDMSOTargetMol | reagent ( 母液浓度为 40 mg/mL ), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:50μLDMSOTargetMol | reagent 母液,添加 300 μLPEG300TargetMol | reagent 混匀澄清,再加 50μLTween 80, 混匀澄清,再加 600μLddH2OTargetMol | reagent 混匀澄清

以上为“体内实验配液计算器”的使用方法举例,并不是具体某个化合物的推荐配制方式,请根据您的实验动物和给药方式选择适当的溶解方案。

1 请输入动物实验的基本信息
mg/kg
g
μL
2 请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
%
%Tween 80
%ddH2O

剂量转换

对于不同动物的给药剂量换算,您也可以参考 更多

关键词

评论列表

4个月前
5.0
Rating icon 很棒

评论内容

Related Tags: buy MK-0752 | purchase MK-0752 | MK-0752 cost | order MK-0752 | MK-0752 chemical structure | MK-0752 in vivo | MK-0752 in vitro | MK-0752 formula | MK-0752 molecular weight