Antifungal agent 38 is a geterocyclic disulfide with antifungal and antibacterial activity. Antifungal agent 38 induces the shrinkage of hyphae, disrupts the integrity of the plasma membrane, and causes the damage and leakage of cell contents [1].

Antifungal agent 38 (50 μg/mL) has anti-fungal activity against 10 pathogenic fungi with inhibition rates of 56.57% ( R. solani ), 100% ( S. sclerotiorum ), 55.09% ( B. cinerea ), 71.23% ( F. graminearum ), 37.32% ( M. oryzae ), 50.87% ( P. capsici ), 100% ( A. flavus ), 59.75% ( P. expansum ), 100% ( M. fructicola ), 65.78% ( R. stolonifer ), respectively [1]. Antifungal agent 38 (5 μg/mL) damages plasma membrane integrity of M. fructicola [1]. Antifungal agent 38 (100 μg/mL) displays excellent anti-bacterial activity against X. oryzae accc 11602, X. axonpodis [1]. Antifungal agent 38 inhibits fungus growth with EC 50 s of 17.09 μg/mL ( R. solani ), 6.34 μg/mL ( S. sclerotiorum ), 25.24 μg/mL ( F. graminearum ), >10 μg/mL ( A. flavus ), 10.42 μg/mL ( M. fructicola ), 45.71 μg/mL ( B. cinerea ), respectively [1]. Cell Viability Assay [1] Cell Line: M. fructicola Concentration: 10, 25, 50 μg/mL Incubation Time: 2, 4, 6 days Result: Inhibited M. fructicola growth.

Antifungal agent 38 (compound S8) (200 μg/mL) shows equivalent curative and higher protective effects as the positive drug Thiophanate-Methyl in vivo [1]. Antifungal agent 38 (50, 100, 200 μg/mL, 30 mL; sprinkling) applies on pears and inhibits M. fructicola growth in vivo [1].