BODIPYFL VH032 is a high-affinity VHL fluorescent probe (Kd= 3.01 nM) with application in time-resolved fluorescence resonance energy-transfer (TR-FRET) assays for high-throughput identification and characterization of VHL ligands. BODIPYFL VH032 consists of a derivative of von Hippel-Lindau (VHL) ligand VH 032 and a fluorophore BODIPYFL(with excitation peak at 504 nm and emission peak at 520 nm), joined by a polyethylene glycol (PEG) 4 linker. The BODIPYFL VH032 VHL TR-FRET signal is stable through the 90 - 300 min incubation time, and theTR-FRET binding assay is sensitive, selective and resistant to assay interference. BODIPYFL VH032 also exhibits high VHL affinity in a fluorescence polarization (FP) assay. Excitation maximum ~ 504 nm; emission maximum ~ 520 nm.
BODIPYFL C5 is a green fluorescent fatty acid that can be used as a precursor for synthesizing various fluorescent phospholipids. It is relatively insensitive to the environment and fluoresces in both water-soluble and lipid environments [1].
BODIPYFL DHPE, a green-fluorescent phospholipid probe, specifically targets and labels the lipid 1,2-dihexadecanoyl-sn-glycero-phosphoethanolamine (DHPE). When labeled, these liposomes can subsequently be internalized through membrane fusion. This compound is particularly useful for studying both membrane surfaces and the process of membrane fusion. (λ ex =505 nm, λ em =511 nm) [1].