Bathophenanthroline disulfonic acid sodium, a versatile dye, plays a crucial role across various domains, from basic scientific research to clinical diagnostics. This compound aids in observing and analyzing cell structures, tracking biomolecules, evaluating cellular functions, differentiating cell types, detecting biomolecules, examining tissue pathology, and monitoring microorganisms. Its usage extends beyond the confines of biological experiments into traditional arenas such as textile dyeing, as well as emerging areas like functional textile processing, food pigments, and dye-sensitized solar cells.