TIGIT Protein, Human, Recombinant (hFc), Biotinylated

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SPR兼容缓冲液

产品编号 TMPY-05121

TIGIT Protein, Human, Recombinant (hFc), Biotinylated is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 39.2 kDa and the accession number is Q495A1-1.

TIGIT Protein, Human, Recombinant (hFc), Biotinylated

一键复制产品信息

SPR兼容缓冲液

产品编号 TMPY-05121

TIGIT Protein, Human, Recombinant (hFc), Biotinylated is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 39.2 kDa and the accession number is Q495A1-1.

规格	价格	库存
5 μg	¥ 693	6-8日内发货
10 μg	¥ 1,170	6-8日内发货
20 μg	¥ 1,950	5日内发货
50 μg	¥ 3,200	5日内发货
100 μg	¥ 5,350	5日内发货

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产品信息

生物活性	Immobilized human CD155-Fc at 2 μg/mL (100 μL/well) can bind TIGIT hFc, Biotinylated, the EC50 of TIGIT-hFc, Biotinylated is 10-60 ng/mL.
产品描述	TIGIT Protein, Human, Recombinant (hFc), Biotinylated is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 39.2 kDa and the accession number is Q495A1-1.
种属	Human
表达系统	HEK293 Cells
标签	C-hFc
蛋白编号	Q495A1-1
别名	WUCAM,VSTM3,VSIG9,T cell immunoreceptor with Ig and ITIM domains
蛋白构建	A DNA sequence encoding the TIGIT (NP_776160.2) (Met1-Pro141) was expressed with the Fc region of human IgG1 at the C-terminus. The purified protein was biotinylated in vitro. Predicted N terminal: Met 22
蛋白纯度	> 95 % as determined by SDS-PAGE.
分子量	39.2 kDa (predicted)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
蛋白性状	Lyophilized powder
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing Sterile PBS. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	TIGIT, also known as V-set and transmembrane domain-containing protein 3 (VSTM3) or V-set and immunoglobulin domain-containing protein 9 (VSIG9) is a new surface protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on regulatory, memory, activated T cells and NK cells. It binds PVR with high affinity, and PVRL2 with lower affinity, but not PVRL3. Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses, however, TIGIT inhibits NK cytotoxicity directly through its ITIM. TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-1 and diminished the production of IL-12p4. Also, TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an "alternative self" mechanism for MHC class I inhibition.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint TargetsImmunotherapyTargeted Therapy