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MERS-CoV Nucleocapsid Protein (His)

产品编号 TMPY-03423

MERS-CoV Nucleocapsid Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 46.51 kDa and the accession number is K0BVN3.

MERS-CoV Nucleocapsid Protein (His)

MERS-CoV Nucleocapsid Protein (His)

产品编号 TMPY-03423
MERS-CoV Nucleocapsid Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 46.51 kDa and the accession number is K0BVN3.
规格价格库存数量
100 μg¥ 5,770现货
200 μg¥ 9,8405日内发货
500 μg¥ 19,9005日内发货
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产品信息

生物活性
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
MERS-CoV Nucleocapsid Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 46.51 kDa and the accession number is K0BVN3.
种属
MERS-CoV
表达系统
Baculovirus Insect Cells
标签C-His
蛋白编号K0BVN3
别名
Nucleo,NP Protein, MERS-CoV,novel coronavirus Nucleoprotein Protein, MERS-CoV,ncov NP Protein, MERS-CoV,cov np Protein, MERS-CoV,coronavirus Nucleoprotein Protein, MERS-CoV,coronavirus Nucleocapsid Protein, MERS-CoV,coronavirus NP Protein, MERS-CoV
蛋白构建
A DNA sequence encoding the MERS-CoV Nucleoprotein (AFS88943.1) (Met1-Asp413) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Met 1
蛋白纯度
> 90 % as determined by SDS-PAGE
MERS-CoV Nucleocapsid Protein (His)
分子量46.51 kDa (predicted)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, 10% gly, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

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