Lysenin Protein, Eisenia fetida, Recombinant (His)

Lysenin Protein, Eisenia fetida, Recombinant (His) is expressed in E. coli expression system with C-6xHis tag. The predicted molecular weight is 40.4 kDa and the accession number is O18423.

规格	价格/CNY		数量
20 μg	￥ 2,290	20日内发货
100 μg	￥ 4,320	20日内发货
1 mg	￥ 14,600	20日内发货

规格

价格/CNY

货期

数量

20 μg

￥ 2,290

20日内发货

100 μg

￥ 4,320

20日内发货

1 mg

￥ 14,600

20日内发货

产品描述	Lysenin Protein, Eisenia fetida, Recombinant (His) is expressed in E. coli expression system with C-6xHis tag. The predicted molecular weight is 40.4 kDa and the accession number is O18423.

种属	Eisenia fetida
表达系统	E. coli
标签	C-6xHis
蛋白编号	O18423
别名	Beta-barrel pore-forming toxin, Invertebrate cytolysin pore, Lysenin, efL1
氨基酸序列	MSAKAAEGYEQIEVDVVAVWKEGYVYENRGSTSVDQKITITKGMKNVNSETRTVTATHSIGSTISTGDAFEIGSVEVSYSHSHEESQVSMTETEVYESKVIEHTITIPPTSKFTRWQLNADVGGADIEYMYLIDEVTPIGGTQSIPQVITSRAKIIVGRQIILGKTEIRIKHAERKEYMTVVSRKSWPAATLGHSKLFKFVLYEDWGGFRIKTLNTMYSGYEYAYSSDQGGIYFDQGTDNPKQRWAINKSLPLRHGDVVTFMNKYFTRSGLCYDDGPATNVYCLDKREDKWILEVVG
蛋白构建	1-297 aa
蛋白纯度	> 90% as determined by SDS-PAGE.
分子量	40.4 kDa (predicted)

缓冲液	If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
复溶方法	Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景	Pore-forming toxin that defensively acts against parasitic microorganisms by forming pores in sphingomyelin-containing membranes. Has hemolytic activity and is also cytotoxic to spermatozoa of some species of invertebrates and many species of vertebrates and to amphibian larvae, guinea pig polymorphonuclear leukocytes, chicken fibroblasts, normal spleen cells and various tumor cells. Is lethal for various species of reptiles, amphibian, birds and mammals. Induces smooth muscle contraction. It binds sphingomyelin and induces hemolysis in the same manner as lysenin-related protein 2, and is 10-fold more effective than lysenin-related protein 1.

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Lysenin Protein, Eisenia fetida, Recombinant (His)

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