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Human respiratory syncytial virus (RSV) (A2) Prefusion glycoprotein F/RSV-preF Protein is expressed in CHO mammalian cells. The predicted molecular weight is 54.5 kDa.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
100 μg | ¥ 6,390 | 5日内发货 |
生物活性 | 1. Immobilized Human respiratory syncytial virus (RSV) (A2) Prefusion glycoprotein F/ RSV-preF Protein at 0.5μg/mL (100μL/well) can bind RSV specific antibody which targets antigenic site II, the EC50 is 0.5 ng/mL-1.7 ng/mL.
2. Immobilized Human respiratory syncytial virus (RSV) (A2) Prefusion glycoprotein F/ RSV-preF Protein at 0.5μg/mL (100μL/well) can bind RSV specific antibody which targets antigenic Site Ø , the EC50 is 0.45 ng/mL-1.35 ng/mL (Routinely tested).
3. Immobilized Human respiratory syncytial virus (RSV) (A2) Prefusion glycoprotein F/ RSV-preF Protein at 0.5μg/mL (100μL/well) can bind prefusion specific antibody which targets antigenic Site V, the EC50 is 0.05 ng/mL-0.15 ng/mL(Routinely tested). |
产品描述 | Human respiratory syncytial virus (RSV) (A2) Prefusion glycoprotein F/RSV-preF Protein is expressed in CHO mammalian cells. The predicted molecular weight is 54.5 kDa. |
种属 | RSV |
表达系统 | CHO Cells |
标签 | Tag Free |
别名 | HRSVgp8,F Protein |
蛋白纯度 | ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. |
分子量 | 54.5 kDa (predicted); 57.6 kDa (reducing conditions) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. It is classified within the genus pneumovirus of the family paramyxoviridae. Like other members of the family, HRSV has two major surface glycoproteins (G and F) that play important roles in the initial stages of the infectious cycle. The G protein mediates attachment of the virus to cell surface receptors, while the F protein promotes fusion of the viral and cellular membranes, allowing entry of the virus ribonucleoprotein into the cell cytoplasm. The fusion (F) protein of RSV is synthesized as a nonfusogenic precursor protein (F), which during its migration to the cell surface is activated by cleavage into the disulfide-linked F1 and F2 subunits. This fusion is pH independent and occurs directly at the outer cell membrane, and the F2 subunit was identifed as the major determinant of RSV host cell specificity. The trimer of F1-F2 interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and induces the fusion between host cell and virion membranes. Notably, RSV fusion protein is unique in that it is able to interact directly with heparan sulfate and therefore is sufficient for virus infection. Furthermore, the fusion protein is also able to trigger p53-dependent apoptosis. |
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