HER2/ERBB2 Protein, Human, Recombinant (aa 23-652, hFc) is expressed in HEK293 mammalian cells with C-Fc tag. The predicted molecular weight is 120-145 KDa and the accession number is P04626.

Human

HEK293 Cells

Tyrosine kinase-type cell surface receptor HER2,TKR1,Receptor tyrosine-protein kinase erbB-2,Proto-oncogene Neu,NGL,MLN19,Metastatic lymph node gene 19 protein,ERBB2,EGFR2

Thr23-Thr652

Thr23-Thr652

Greater than 95% as determined by reducing SDS-PAGE. Greater than 95% as determined by SEC-HPLC.

Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Human epidermal growth factor receptor 2 (HER2) is a type of membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. HER2 plays a key role in development, cell proliferation and differentiation. HER2 has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on. HER2 is activated by dimerization and not activated by EGF, TGF-alpha and amphiregulin. Interaction with PTK6 increases its intrinsic kinase activity.It is heterodimer with EGFR, ERBB3 and ERBB4. HER2 associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. It implicated in transcriptional activation of CDKN1A and the function of the protein involves STAT3 and SRC. And also it involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.