ECM1 Protein, Mouse, Recombinant (His)

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SPR兼容缓冲液

产品编号 TMPY-01908

ECM1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 62.5 kDa and the accession number is Q61508-1.

ECM1 Protein, Mouse, Recombinant (His)

一键复制产品信息

SPR兼容缓冲液

产品编号 TMPY-01908

ECM1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 62.5 kDa and the accession number is Q61508-1.

规格	价格	库存
5 μg	¥ 428	6-8日内发货
10 μg	¥ 683	6-8日内发货
20 μg	¥ 1,130	5日内发货
50 μg	¥ 2,270	5日内发货
100 μg	¥ 4,430	5日内发货

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产品信息

生物活性	Measured by the ability of the immobilized protein to support the adhesion of HFF Human skin fibroblast cells. When 5 × 10E4 cells/well are added to mECM1-His coated plates (2.5 μg/mL and 100 μL/well), approximately > 45 % will adhere specifically after 90 minutes at 37 ℃.
产品描述	ECM1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 62.5 kDa and the accession number is Q61508-1.
种属	Mouse
表达系统	HEK293 Cells
标签	C-His
蛋白编号	Q61508-1
别名	p85,extracellular matrix protein 1,AI663821
蛋白构建	A DNA sequence encoding the mouse ECM1 isoform 1 (Q61508-1) (Met 1-Glu 559) was expressed, fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Ala 20
蛋白纯度	> 88 % as determined by SDS-PAGE
分子量	62.5 kDa (predicted); 90-95 kDa (reducing condition, due to glycosylation)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
蛋白性状	Lyophilized powder
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Extracellular matrix protein 1 (ECM1) is a secreted glycoprotein and playing a pivotal role in endochondral bone formation, angiogenesis, and tumour biology. Three splice variants have been identified: ECM1a (540 aa) is most widely expressed, with highest expression in the placenta and heart; ECM1b (415 aa) is differentiation-dependent expressed and found only in tonsil and associated with suprabasal keratinocytes; ECM1c (559 aa) accounts for approximately 15% of skin ECM1. Although ECM1 is not tumor specific, is significantly elevated in many malignant epithelial tumors and is suggested as a possible trigger for angiogenesis, tumor progression and malignancies. It also has been shown to regulate endochondral bone formation, skeletal development and tissue remodeling.