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规格 | 价格 | 库存 | 数量 |
---|---|---|---|
I+II | ¥ 360 | 现货 | |
(I+II)*10 | ¥ 2,640 | 现货 | |
(I+II)*50 | ¥ 10,360 | 现货 | |
(I+II)*100 | ¥ 15,200 | 现货 |
管号 | Ingredient | CAS | MW | Concentration (100x) | Target | Type |
---|---|---|---|---|---|---|
I (ddH2O) 1mL | Sodium Fluoride | 7681-49-4 | 41.99 | 100 mM | Acid Phosphatase | Reversible |
Sodium Orthovanadate | 13721-39-6 | 183.91 | 100 mM | Alkaline phosphatase, PTPs, ATPases | Reversible | |
Sodium Molybdate | 7631-95-0 | 205.92 | 115 mM | Acid and phosphoprotein Phosphatase | Irreversible | |
Sodium Tartrate | 868-18-8 | 194.05 | 400mM | Acid Phosphatase | Reversible | |
Imidazole | 288-32-4 | 68.08 | 200 mM | Alkaline phosphatase | Reversible | |
II (DMSO) 1mL | (-)-p-Bromotetramisole oxalate | 62284-79-1 | 373.22 | 2.5 mM | Alkaline phosphatases | Irreversible |
Cantharidin | 56-25-7 | 196.2 | 500 μM | Ser/Thr phosphatases | Reversible | |
Microcystin LR | 101043-37-2 | 995.17 | 500 nM | Acid and PP1 and PP2A | Reversible |
在室温下解冻(如果DMSO在室温下融化较慢,可考虑25℃水浴融化),开盖前请低速离心一下,以便将黏附于管壁的液体甩至管底。然后在实验前以1:100(v / v)稀释至溶液样品(如细胞裂解液或组织提取液),短暂涡旋震荡加速溶解。应用于:WB、IP、Co-IP、pull-down、IF、IHC、kinase assay等。
Attribute | Value |
---|---|
Form | Liquid |
Formulation | 2 Tube Solution (100×) |
Storage | -20 ℃ for one year, Avoid free/thaw |
Freeze | Ok to freeze |
Toxicity | Irritant |
注意如果将磷酸酶抑制剂Cocktail I 与Cocktail II一起使用,要先加Cocktail I于样品液中混匀,然后再加Cocktail II,再次混匀,不可将Cocktail I 与Cocktail II预先混匀,可能会产生沉淀,因此要分步加入。
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