Artemisinin (Qinghaosu) is a natural product, a sesquiterpene lactone, derived from Artemisia annua. Artemisinin has anti-malarial activity, as well as neuroprotective and anti-tumor activity.

方法：神经母细胞瘤细胞 SK-N-AS、BE(2)-C、SK-N-DZ 和 SHEP1 用 Artemisinin (100-400 µM) 处理 1-7 天，通过 CCK-8 assay 测定细胞活力。
结果：观察到神经母细胞瘤细胞对 Artemisinin 的浓度和时间依赖性反应，Artemisinin 显著抑制神经母细胞瘤细胞的增殖。[1]
方法：葡萄膜黑色素瘤 UM 细胞用 Artemisinin (3.125-100 µM) 处理 24 h，通过 Western Blot 检测靶点蛋白表达水平。
结果：Artemisinin 以剂量依赖的方式显著抑制 PI3K、AKT 和 mTOR 的磷酸化。[2]

方法：为检测体内抗肿瘤活性，将 Artemisinin (100 mg/kg) 腹腔注射给携带 BE（2）-C 异种移植物的 NOD/SCID 小鼠，每天一次，持续 12 天。
结果：Artemisinin 治疗组异种移植物肿瘤的体积和重量比 DMSO 组小得多，也轻得多。Artemisinin 显著抑制神经母细胞瘤细胞的致瘤性。[1]

Briefly, PC12 cells are lysed in lysis buffer and centrifuged at 12,500×g for 5 min 15 mL of cell lysate is incubated with 50 mL of 2X substrate working solution at room temperature for 30 min in 96-well plates. The fluorescence intensity is then determined by Infinite M200 PRO Multimode Microplate at an excitation wavelength of 490 nm and emission at 520 nm. The fluorescence intensity of each sample is normalized to the protein concentration of sample. All values of % caspase 3/7 activities are normalized to the control group.

Artemisinin is diluted in 0.01% DMSO. For this purpose, cells are cultivated in 96-well plates in DMEM, supplemented with insulin. The artemisinin, Dox, and DDP are added to media at different concentrations and the cells are cultivated for either 24 or 48 h. For this purpose artemisinin is diluted in 0.01% DMSO in media. After this time, 10 μL of the MTT dye solution (5 mg/mL in phosphate buffer saline) is added to the cells; the cells are incubated at the same conditions for 3 h. After centrifugation (1500 rpm, 5 min) the supernatant is removed. 100 μL of dimethyl sulfoxide is added to each well, to dissolve formazan. The absorption is measured, using a multi-well spectrophotometer at a wavelength of 540 nm.