NanoLuc substrate 1 is a versatile dye utilized in biological experiments. It aids researchers in visualizing and analyzing cell structures, tracking biomolecules, assessing cell functions, distinguishing cell types, detecting biomolecules, studying tissue pathology, and monitoring microorganisms. Its applications extend from fundamental scientific research to diverse clinical diagnostics. Dyes are also prevalent in traditional areas like textile dyeing and innovative fields such as functional textile processing, food pigments, and dye-sensitized solar cells.