Gossypol acetic acid (AT101), a polyphenolic compound isolated from cottonseeds, binds with Bcl-2, Bcl-xL, Mcl-1, and does not inhibit BIR3 domain and BID.

BCL2:0.2-0.3 mM (Ki), BCL-XL:0.5-0.6 μM (Ki)

Gossypol，一种已知的来自棉属植物Gossypium的抗精子生成剂，在剂量依赖的方式下被发现能够抑制黄鲈鱼精子的体外运动能力以及精子中乳酸脱氢酶的活性[1]。此外，Gossypol还被证实对某些类型的癌细胞系具有体外抗增殖和诱导凋亡的作用[2]。

Gossypol acetate 能够抑制带有Wus1的小鼠的肿瘤生长，但并不延长小鼠的生存时间，且在短暂抑制后肿瘤迅速增长。Gossypol已被发现在卵巢癌、子宫内膜癌、肾上腺皮质瘤、甲状腺癌、肺癌、结肠癌、白血病、胰腺癌、黑色素瘤及淋巴瘤等方面具有抑制增殖或诱导凋亡的作用。此外，gossypol能增加药物抵抗性肿瘤细胞对化疗和放疗的敏感性。一些临床试验表明，gossypol具有良好的耐受性，并在一些患者中观察到部分反应。

MM(Multiple myeloma) cells are plated in 24-cell culture clusters at a density of 1x105 viable cells/l per well. Triplicate wells are treated with 1, 5, 10, 25 and 50 µmol/l gossypol acetate, and the negative control group is supplemented with 0.1% DMSO. Then, cell numbers at different treatment time points (0, 24, 48 and 72 h) are determined by using a hemocytometer and the trypan blue dye-exclusion method. The trypan blue dye-exclusion method is used to evaluate the cell viability. The cells are examined in a counting chamber under a light microscope. Only viable cells are recorded. (Only for Reference)