Gossypol acetic acid (AT101), a polyphenolic compound isolated from cottonseeds, binds with Bcl-2, Bcl-xL, Mcl-1, and does not inhibit BIR3 domain and BID.

BCL-XL:0.5-0.6 μM (Ki), Bcl-2:0.2-0.3 mM (Ki)

MM(Multiple myeloma) cells are plated in 24-cell culture clusters at a density of 1x105 viable cells/l per well. Triplicate wells are treated with 1, 5, 10, 25 and 50 µmol/l gossypol acetate, and the negative control group is supplemented with 0.1% DMSO. Then, cell numbers at different treatment time points (0, 24, 48 and 72 h) are determined by using a hemocytometer and the trypan blue dye-exclusion method. The trypan blue dye-exclusion method is used to evaluate the cell viability. The cells are examined in a counting chamber under a light microscope. Only viable cells are recorded. (Only for Reference)

乙酸棉子酚, 醋酸棉酚, Pogosin, Gossypol acetic acid, AT101, (±)-Gossypol-acetic acid

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 100 mg/mL (172.83 mM), Sonication is recommended.

Ethanol: 2 mg/mL (3.46 mM), Sonication is recommended.

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4 mg/mL (6.91 mM), Sonication is recommended.