规格

价格

库存

数量

1 mg

¥ 442

现货

2 mg

¥ 648

现货

5 mg

¥ 1,090

现货

10 mg

¥ 1,530

现货

25 mg

¥ 2,510

现货

50 mg

¥ 3,580

现货

100 mg

¥ 4,910

现货

200 mg

¥ 6,480

现货

1 mL x 10 mM (in DMSO)

¥ 1,070

现货

生物活性

产品描述	Nesbuvir is a selective hepatitis C virus (HCV) nonstructural protein 5B (NS5B) RNA-dependent polymerase inhibitor.Nesbuvir has an IC50 of 9 nM against hepatocellular carcinoma cells containing 1b HCV replicons.
靶点活性	NS3V170A:13 nM (EC50), NS5B:5 nM, NS3V170A:9 nM (EC50), NS3K583T:15 nM (EC50), NS5BI424V:13 nM (EC50)
体外活性	Nesbuvir (40 or 80 nM) (approximately 10 and 20 times the EC50 in a 3-day replicon inhibition assay, respectively). The EC50 for Nesbuvir in the transient expression assay is 14 nM, whereas it is 5 nM for the stable replicon.[1] Nesbuvir (0.1 and 1 μM; Huh-7 cells; for 16 days) reduced about 3.6 log10 and 4.2 log10 HCV RNA levels, respectively.[3]
体内活性	Nesbuvir is demonstrated to yield significant antiviral effects in mice with chimeric human livers and in patients infected with HCV.[2] Nesbuvir (chimeric mouse model) treatment resulted in a 2.02 +/- 0.55 log reduction in HCV titer, whereas in combination with interferon using a suboptimal dose of 30 mg/kg three times per day showed a 2.44 log reduction and were better than interferon treatment only.[5]
细胞实验	Huh7-BB7 cells are seeded at a density of 20,000 cells per 100 mm dish in DMEM supplemented with 2% FBS, 1 mg/mL G418, and various concentrations of Nesbuvir and/or Boceprevir with DMSO at a final concentration of 0.5% (vol/vol). The medium is removed and is replaced with a fresh medium with the appropriate compound concentrations every 3 or 4 days. After 7 days, the cells are split 1 to 10, placed into fresh 100 mm dishes, and incubated with medium with the appropriate compound concentrations. After 20 days, the medium is removed and the cells are fixed with 7% (wt/vol) formaldehyde and stained with 1% (wt/vol) crystal violet in 50% (vol/vol) ethanol [1].
别名	HCV-796

化学信息

分子量	446.49
分子式	C₂₂H₂₃FN₂O₅S
CAS No.	691852-58-1
Smiles	CNC(=O)c1c(oc2cc(N(CCO)S(C)(=O)=O)c(cc12)C1CC1)-c1ccc(F)cc1
密度	1.407 g/cm3

储存&溶解度

存储

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

溶解度信息

DMSO: 45 mg/mL (100.79 mM), Sonication is recommended.

体内实验配方

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (4.48 mM), Sonication is recommended.

请按顺序添加溶剂，在添加下一种溶剂之前，尽可能使溶液澄清。如有必要，可通过加热、超声、涡旋处理进行溶解。工作液建议现配现用。以上配方仅供参考，体内配方并不是绝对的，请根据不同情况进行调整。

溶液配制表

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.2397 mL	11.1985 mL	22.3969 mL	111.9846 mL
5 mM	0.4479 mL	2.2397 mL	4.4794 mL	22.3969 mL
10 mM	0.2240 mL	1.1198 mL	2.2397 mL	11.1985 mL
20 mM	0.1120 mL	0.5599 mL	1.1198 mL	5.5992 mL
50 mM	0.0448 mL	0.2240 mL	0.4479 mL	2.2397 mL
100 mM	0.0224 mL	0.1120 mL	0.2240 mL	1.1198 mL