Ribavirin (Tribavirin) is a synthetic nucleoside analog of ribofuranose with activity against hepatitis C virus and other RNA viruses.

ALT、AST活性和胆红素水平在联合使用JAT、干扰素和Ribavirin 的情况下显著降低(p<0.01)。JAT、干扰素或Ribavirin 单独与CCl4共用时，肝脏似乎显示出对CCl4的某种保护作用，表现为肝细胞索的正常结构、坏死的缺席和较少的脂肪浸润。分别或联合使用JAT、Peg-干扰素和Ribavirin 的各组显示TGF-β和Bax表达减少。在联合使用干扰素、Ribavirin 和JAT的组中，p53的表达水平显著降低[6]。用Ribavirin 胶囊(400 mg)治疗的Wistar大鼠，血清和肝脏水平的活化素A显著减少，而抑制素显著增加。当Ribavirin 与IFN-α或Peg-IFN-α联合使用时，Ribavirin 显示出强大的抗病毒活性[7]。Ribavirin (40 mg/kg，口服)显著提高了CM-10-18在小鼠中的抗病毒功效。虽然Ribavirin 能抑制培养细胞中的登革热病毒感染，但在单一化合物治疗中它在减少病毒血症方面无效[8]。

方法：为研究对 CCHF 病毒的抗病毒效果，将 Ribavirin (100 mg/kg) 腹腔注射给 CCHF 病毒感染的 IFNAR-/- 小鼠，每天一次，持续到死亡或第 8 天。
结果：CCHF 病毒感染的 IFNAR-/- 小鼠在感染后 2-6 天死亡，血液和器官中转氨酶水平升高，病毒滴度高。Ribavirin 没有增加 IFNAR-/- 小鼠的存活率，但延长了死亡时间，降低了转氨酶水平和病毒滴度。[3]

The effect of Ribavirin on microglial cell viability is evaluated by the sulforhodamine B (SRB) chemosensitivity assay. Briefly, LPS-stimulated microglial cells are incubated for 48 h in the presence or absence of Ribavirin. Afterward, the cells are fixed in 10% (w/v) trichloroacetic acid for 1 h at 4°C, rinsed in tap water and stained with 0.4% (w/v) SRB in 1% acetic acid (100 μL/well) for 30 min at room temperature (RT). The cells are then rinsed three times in 1% acetic acid to remove the unbound stain. The protein bound stain is extracted with 200 μL 10 mM Tris base (pH 10.5) per well. The optical density is read at 540 nm, with correction at 670 nm. The results are presented as percentage of the control (non-stimulated/untreated microglial cells), that is arbitrarily set to 100%.