cell viability

2-NBDG 是一种荧光指示剂 (λex=467 nm，λem=542 nm)，是一种荧光 D-葡萄糖类似物。2-NBDG 可用于监测活细胞和组织中的葡萄糖吸收，可以通过实时共聚焦、高分辨率或宽视场荧光显微镜和流式细胞术进行监测。

4-Di-1-ASP是一种苯乙烯基染料，适用于标记活脑组织中的神经胶质瘤细胞，常用于分析细胞的结构、活性、增殖以及内吞作用、胞吐和吞噬作用。同时，该染料也被用于观察活细胞的线粒体结构。在显微镜观察下，4-Di-1-ASP能够发射绿色荧光（λex λem= 475 606 nm）。

Firefly luciferase mRNA可编码一种起源于Photinus pyralis（萤火虫）的荧光素酶。作为广泛运用于基因调控和功能研究的生物发光报告系统，Firefly luciferase适合进行基因表达、细胞活性以及活体成像等各类检测分析。

CytoCalcein Violet 500 is a fluorogenic dye used to assess cell viability. It is a cell-permeable dye that, upon entering cells, is cleaved by intracellular esterases. CytoCalcein Violet 500 is retained by living cells and displays excitation/emission maxima of 405/500 nm, respectively.

Calcein Red AM is a fluorogenic dye that is used to assess cell viability. As the acetoxymethyl ester, Calcein Red AM is non-fluorescent but cell-permeant and is hydrolyzed to the fluorescent probe Calcein Red by intracellular esterases. Calcein Red is retained by living cells and displays excitation/emission maxima of 560/574 nm, respectively. Calcein Red AM is a red-emitting variant of the green fluorophore calcein AM , which has been used to evaluate cell viability and cytotoxicity.

Calcein-AM is a cell-permeable fluorescent dye used for the determination of cell viability.

CytoCalcein Violet 450 is a fluorogenic dye used to assess cell viability. It is a cell-permeable dye that, upon entering cells, is cleaved by intracellular esterases. CytoCalcein Violet 450 is retained by living cells and displays excitation emission maxima of 405 450 nm, respectively.

5(6)-CFDA (5-(6)-Carboxyfluorescein diacetate) 是具有细胞渗透性的胺反应型绿色荧光探针(λex: 492 nm; λem: 517 nm)，可用于细胞染色分析。

Calcein Orange Diacetate is a fluorogenic dye that is used to assess cell viability. It is hydrolyzed to the fluorescent probe Calcein Orange . Calcein Orange is retained by living cells and displays excitation emission maxima of 525 550 nm, respectively. Calcein Orange Diacetate is an orange-emitting variant of the green fluorophore Calcein AM , which has been used to evaluate cell viability and cytotoxicity.

EthD-1 is a cell-impermeant viability indicator. It acts as a high-affinity nucleic acid stain.

Bromocresol green是一种pH敏感的三苯甲烷染料，专用于测定蛋白质及血清中的白蛋白浓度。此生物碱性染料在pH值3.0至7.5的范围内颜色从黄绿到蓝绿变化。在酸性条件下（例如pH=4.15），Bromocresol green显黄色（λmax=435 nm，质子化形式），而在碱性环境下则转为蓝色（λmax=615 nm，去质子化形式）。因此，它被广泛用作pH指示剂，在生化分析中有着重要应用。Bromocresol green还用于测定肌酐等分子浓度及评估细胞存活率。

Resazurin is used as an oxidation-reduction indicator in cell viability.

eGFP circRNA-LNP 是一种含有eGFP circRNA 的脂质纳米粒，可用于RNA传递、翻译效率和细胞活力检测。该eGFP circRNA包括增强型绿色荧光蛋白标签，为细胞内绿色荧光蛋白的表达提供基础，且eGFP作为报告基因，可通过荧光显微镜或流式细胞术进行检测。

eGFP mRNA-LNP 是一种蕴含eGFP mRNA 的脂质纳米粒，应用于RNA传递、翻译效率及细胞活力的测定。eGFP circRNA携带eGFP以实现绿色荧光蛋白表达，此蛋白可经由荧光显微镜或流式细胞术进行检测，充当报告基因。

Calcein tetraethyl ester, a fluorescent dye, is utilized in biological studies for its ability to penetrate cellular membranes into living cells. This property renders it valuable for assessing cell viability and short-term cell labeling.