4-Di-1-ASP, a styryl dye, selectively stains glioma cells within live brain tissue, facilitating microscopic analysis of cell structure, viability, and processes including proliferation, endocytosis, cytokinesis, and phagocytosis. It also allows for the observation of mitochondrial structures in living cells. The dye exhibits green fluorescence when visualized under a microscope (λ ex /λ em = 475/606 nm) [1] [2].

依照指南（以下是我们推荐的操作步骤。此步骤仅供参考，请根据具体需求进行调整）[1]。首先，将染料溶解在水中配制成2 mM的母液。其次，将准备好的细胞切片放置于35°C的恒温箱中，加入配制好的染料溶液至最终浓度达1 μM，并与染料共孵育10分钟。最后，将染色后的细胞切片置于显微镜载玻片上，覆盖盖玻片，并利用荧光共聚焦显微镜在最大激发波长475 nm及最大发射波长606 nm下进行观察。