GJ103 sodium salt is an active analog of GJ072, a read-through compound. It has been shown to reduce the surface tension of aqueous solutions, which makes it easier for molecules to move through the solution. It has also been shown to reduce the viscosity of aqueous solutions, which makes it easier for molecules to move through the solution. Additionally, it has been shown to reduce the pH of aqueous solutions, which can have a variety of effects on the biochemical and physiological processes of living organisms.

GJ072在TGA和TAG终止密码子上激活ATM激酶，并通过FACS检测恢复了ATMpSer1981自磷酸化和SMC1pSer966跨磷酸化。GJ072对具有同源TAA突变的A-T细胞活跃，并能在经治疗的A-T细胞中诱导检测到的完整长度ATM蛋白。初步的构效关系研究产生了八个GJ072的活性类似物。一些GJ072类似物（例如GJ103、GJ106、GJ109和GJ111）通过FCATMpSer1981和IRIF试验，在所有三个PTCs中一致显示其活性。GJ071、GJ072及其一些类似物（如GJ103）与RTC13或RTC14具有相似的读通活性，但对A-T细胞来说比RTC13和RTC14更为耐受。GJ103在高达300 μM的浓度下，不在A-T细胞中显示明显的细胞毒性。

GJ103 sodium salt 具有水溶性，这将使我们能够通过系统给药评估其体内活性。

Cell proliferation assay is used to measure cytotoxicity. Cells are seeded into a flat-bottom 96-well plate, including control wells containing complete growth medium alone as blank absorbance readings. After RTC treatment (GJ103), activated-XTT Solution is added into each well, and the cells are returned to the cell culture incubator for 12-14 hours. The absorbance is measured at 480 nM with relevant 630 nM to assess nonspecific readings.