Antileishmanial agent-17, a coumarin hybrid, exhibits potent antileishmanial activity (IC50 <0.78 μM) while proving non-toxic to normal VERO cells. It specifically interacts with the folate pathway enzymes pteridine reductase and DHFR-TS. Efficacy against the promastigote form is highlighted by an IC50 value of 0.40 μM, and against the amastigote form, an IC50 of 0.68 μM, respectively.

Antileishmanial agent-17（compound 14b）在20 μM和100 μM浓度下，分别对叶酸途径显示82%和91%的抑制效果，同样条件下对亚叶酸的抑制效果为88%和91% [1]。此外，Antileishmanial agent-17在244.3 μM的CC50浓度条件下对非洲绿猴肾细胞（VERO 细胞）展现较低的细胞毒性 [1]。