Anacardic Acid (6-pentadecylsalicylic Acid) is an effective inhibitor of p300 and p300/CBP-associated factor histone acetyltranferases. It also has antimicrobial activity, antibacterial activity, and inhibits prostaglandin synthase, lipoxygenase, and tyrosinase.

PCAF(p300/CREB-binding protein-associated factor):5 μM, p300 HAT:8.5 μM

Anacardic acid作为多个临床靶向酶的抑制剂，例如HATs、NF-kB、LOX-1、黄嘌呤氧化酶、酪氨酸酶和尿素酶，并且在体外也是Tip60的有效抑制剂。它抑制了在HeLa细胞中Tip60依赖的ATM蛋白激酶的乙酰化和激活，从而使细胞对辐射的细胞毒性效应更加敏感。

Anacardic Acid 通过减少暴露于乙醇的胎鼠心脏中Gata4、α-MHC和cTnT的过表达，抑制PCAF、P300与Gata4启动子的结合。

HAT and HDAC Activity Assays :After homogenization of cardiac tissues, nucleoproteins are extracted using a Nuclear Extract Kit according to the manufacturer's instructions. HAT and HDAC activities of the nuclear protein extracts are determined using a colorimetric assay included in the HAT and HDAC assay kits.

HeLa and 293T cells are grown in Dulbecco's modified Eagle's medium/10% fetal calf serum. SQ20B and SCC35 squamous cell carcinoma cell lines are cultured in modified eagles medium/10% fetal calf serum. For anacardic acid (EMD Biosciences, CA) exposure, cells are preincubated with anacardic acid (0-100 μM) for 40 min, irradiated, then allowed to recover for 4 h. Cells are then switched to fresh media and allowed to grow for 10 days.

Animal Models: 9 to 11 week old Kunming mice. Formulation: Dissolved in DMSO,co-injected with ethanol. Dosages: 0,1.25,2.5,5,10 mg/kg. Administration: i.p.