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DMOG (Dimethyloxalylglycine) 是一种 α-酮戊二酸辅因子的拮抗剂,一种 HIF 脯氨酰羟化酶 (HIF-PH) 的抑制剂,可导致蛋白 HIF-1α 的积聚和稳定。DMOG 可以诱导细胞自噬,还是一种促血管生成剂。
DMOG (Dimethyloxalylglycine) 是一种 α-酮戊二酸辅因子的拮抗剂,一种 HIF 脯氨酰羟化酶 (HIF-PH) 的抑制剂,可导致蛋白 HIF-1α 的积聚和稳定。DMOG 可以诱导细胞自噬,还是一种促血管生成剂。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 mg | ¥ 541 | 现货 | |
100 mg | ¥ 837 | 现货 | |
200 mg | ¥ 1,458 | 现货 | |
500 mg | ¥ 3,262 | 现货 |
产品描述 | DMOG (Dimethyloxalylglycine) is an antagonist of α-ketoglutarate cofactor, an inhibitor of HIF prolyl hydroxylase (HIF-PH), which leads to the accumulation and stabilization of the protein HIF-1α. DMOG induces autophagy, and is a pro-angiogenic agent. |
体外活性 | 方法:PC12 细胞在常氧 (21% O2) 用 DMOG (1 µM-2 mM) 处理 24 h,使用 MTT assay 检测细胞活力。 结果:DMOG 引起显著的细胞毒性和活细胞部分的减少。[1] 方法:人肌腱干细胞 hTSC 用 DMOG (0.01-1 mM) 处理 96 h,使用 Western Blot 检测靶点蛋白表达水平。 结果:对 HIF-1α 核定位的分析显示,DMOG 以剂量依赖的方式激活该因子。[2] |
体内活性 | 方法:为检测体内抗炎活性,将 DMOG (8 mg/mouse) 腹腔注射给小鼠,2 h 后注射 LPS (10 mg/kg) 诱导休克。 结果:DMOG 预处理可以减弱 LPS 诱导的 NF-κB 通路的全身激活。此外,用 DMOG 处理的小鼠在 LPS 诱导的休克中的存活率显著增加。[3] |
细胞实验 | To analyze DNA synthesis as an index of cellular proliferation, VSMC are plated in 48-well plates (5,000 per square centimeter) in growth medium, incubated overnight, and serum-deprived (1% FCS) for 24 h. Replicate wells are then stored at −70°C for baseline (day 0) cell counts, and fresh medium with or without growth factors is added to the remaining wells, which are incubated 72-96 h in 20 or 5% O2. Days 0 and 3 or 4 cell counts are determined by lysing cells in a buffer containing a fluorescent dye, which has minimal fluorescence by itself but fluoresces when bound to DNA or RNA. Absolute cell numbers are calculated by comparing the fluorescence of specimens with that of a standard curve similarly prepared using a known number of cells. (Only for Reference) |
别名 | 二甲基草酰甘氨酸, Dimethyloxalylglycine, Dimethyloxaloylglycine, Dimethyloxallyl Glycine |
分子量 | 175.14 |
分子式 | C6H9NO5 |
CAS No. | 89464-63-1 |
Smiles | COC(=O)CNC(=O)C(=O)OC |
密度 | 1.247g/cm3 |
存储 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. | |||||||||||||||||||||||||||||||||||
溶解度信息 | H2O: 17.54 mg/mL (100 mM), Sonication is recommended. DMSO: 60 mg/mL (342.58 mM) Ethanol: 35 mg/mL (199.84 mM) | |||||||||||||||||||||||||||||||||||
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