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CD99 Protein, Rat, Recombinant (hFc)

产品编号 TMPY-03096

别名: CD99 molecule

CD99 Protein, Rat, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 37.6 kDa and the accession number is B4F7A5.

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CD99 Protein, Rat, Recombinant (hFc)

规格	价格/CNY		货期	数量
100 μg	￥ 3,820		5日内发货

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生物活性	Testing in progress
产品描述	CD99 Protein, Rat, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 37.6 kDa and the accession number is B4F7A5.

种属	Rat
表达系统	HEK293 Cells
标签	C-hFc
蛋白编号	B4F7A5
别名	CD99 molecule
蛋白构建	The Rat CD99 (B4F7A5) (Met1-Gly127) was expressed, fused with the Fc region of Human IgG1 at the C-terminus.
蛋白纯度	> 95 % as determined by SDS-PAGE
分子量	37.6 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD99 is a transmembrane protein expressed on most hematopoietic cells, endothelial cells and at the borders between confluent cells. CD99 is also found expressed in the development of normal ovary and testis as well as in 25 sex cord-stromal tumors, 7 epithelial neoplasms, and 6 germ cell tumors. CD99 may be a useful marker for sex cord-stromal tumors and that its degree of reactivity correlates with the degree of differentiation in Sertoli-Leydig cell tumors. Additionally, CD99 might aid in distinguishing granulose cell tumors of the ovary from poorly differentiated carcinomas and it has been reported to be a sensitive and specific marker for Ewing's sarcoma and primitive neuroectodermal tumor.

参考文献

1. Zola H,et al.(2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5. 2. Ho IC,et al.(2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35. 3. Matesanz-Isabel J,et al.(2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12. 4. Schenkel AR,et al.(2002) CD99 plays a major role in the migration of monocytes through endothelial junctions. Nature Immunology. 3: 143 - 50. 5. Gordon MD,et al.(1998) CD99, keratin, and vimentin staining of sex cord-stromal tumors, normal ovary, and testis. Mod Pathol. 11 (8): 769-73.

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Keywords

CD99 Protein, Rat, Recombinant (hFc) CD99 molecule recombinant recombinant-proteins proteins protein

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