CD73 Protein, Cynomolgus, Recombinant (His)

产品编号 TMPY-03377

CD73 Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59.2 kDa and the accession number is EHH53214.1.

CD73 Protein, Cynomolgus, Recombinant (His)

产品编号 TMPY-03377

CD73 Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59.2 kDa and the accession number is EHH53214.1.

规格	价格	库存
50 μg	¥ 3,820	现货
100 μg	¥ 6,390	5日内发货
200 μg	¥ 10,670	5日内发货
500 μg	¥ 20,980	5日内发货

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产品信息

生物活性	Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit . The specific activity is >20,000 pmol/min/µg.
产品描述	CD73 Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59.2 kDa and the accession number is EHH53214.1.
种属	Cynomolgus
表达系统	HEK293 Cells
标签	C-His
蛋白编号	EHH53214.1
别名	5'-nucleotidase, ecto (CD73)
蛋白构建	A DNA sequence encoding the cynomolgus NT5E (EHH53214.1) (Met1-Lys547) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Trp 27
蛋白纯度	> 90 % as determined by SDS-PAGE
分子量	59.2 kDa (predicted); 59 kDa (reducing conditions)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 0.12 M NaCl, 4 mM CaCl2, 20% glycerol, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein that belongs to the 5'-nucleotidase family. CD73 is a glycosylphosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signaling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1, and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purinergic receptors to induce cellular responses. CD73 has also been reported to regulate the expression of pro-inflammatory molecules in mouse endothelium.

计算器

复溶计算器
重组蛋白稀释计算器
比活力计算器

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关键词

CD 73 CD-73 5'-nucleotidase, ecto CD73