Qstatin

QStatin 是一种弧菌物种群体感应的选择性SmcR 抑制剂，EC50值为 208.9 nM。与 SmcR 紧密结合并改变了蛋白质的柔韧性，从而改变了其转录调控活性。并减弱其在水生宿主中的毒力。它可用于水产养殖的可持续性抗弧菌病药物。

产品编号 T5816Cas号 902688-24-8

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纯度:99.99%

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产品编号 T5816Cas号 902688-24-8

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生物活性

产品描述	QStatin, a Selective Inhibitor of Quorum Sensing in Vibrio Species( SmcR with an EC50 of 208.9 nM).
靶点活性	SmcR:208.9 nM(EC50)
体外活性	Qstatin 对SmcR活性的抑制作用最强，而且对V. vulnificus的生长抑制不显著。通过测量不同浓度的QStatin下V. vulnificus WT (pBB1)的RLU水平来评估SmcR活性，结果显示QStatin的半最大有效浓度(EC50)为208.9 nM。
细胞实验	The plasmid pBB1 carrying the LuxRVh homologue-activated lux operon was conjugally transferred into the V. vulnificus wild-type (WT) strain, the ΔsmcR mutant, or the ΔluxO mutant. These V. vulnificus reporter strains were grown overnight, diluted 1:1,000 in fresh LBS, and treated with hit molecules. RLU values were calculated every hour. To determine the EC50, QStatin (10^?12 to 10^?4 M) or 2% DMSO as a control was added to the V. vulnificus WT reporter strain and RLU was measured after 5 h. The percentage of SmcR activity of the sample at a given concentration of QStatin was determined using the following equation: percent SmcR activity = sample RLU/control RLU × 100. The EC50 of QStatin (the concentration reducing the SmcR activity to 50%) was calculated from a plot of the percentages of SmcR activity versus QStatin concentrations using GraphPad Prism 6.0.

化学信息

储存&溶解度

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

溶解度信息

DMSO: 83.33 mg/mL (284.25 mM)

溶液配制表

DMSO