PI3Kα/HDAC6-IN-1 (compound 21j) is a dual inhibitor of PI3Kα and HDAC6, exhibiting IC50 values of 2.9 nM and 26 nM, respectively. It effectively hinders AKT(Ser473) phosphorylation and promotes the build-up of acetylated α-tubulin, while not influencing acetylated histones H3 and H4. The compound demonstrates potent anti-cancer efficacy by inhibiting the L-363 cell line with an IC50 of 0.17 μM [1].