Methylene Violet 3RAX is a cationic dye, a potential photosensitizer in photodynamic therapy, with antitumor activity that alters the molecular structure of DNA, disrupts the modules of DNA, and induces the production of reactive monoclinic oxygen species, which are used to stain the mitochondria of cells.Methylene Violet 3RAX inhibits the subcellular localization of cancer cells by severing the Methylene Violet 3RAX inhibits subcellular localization of cancer cells, leading to cell death by cutting DNA strands in tumor cells.

BChE (human plasma):0.51 μM (Ki), AChE (human erythrocyte):1.58 μM (Ki)

Methylene Violet 3RAX 能够抑制人红细胞 AChE 和人血浆 BChE，其抑制常数（Ki）分别为 1.58 μM 和 0.51 μM。[2] 它还表现出抗肿瘤活性，通过切割肿瘤细胞内的 DNA 链实现作用。在浓度范围 0-400 µM 时，Methylene Violet 3RAX
显著增强了 DNA 的裂解效果。当浓度达到 300 μM 时，其对 DNA 的光裂解活性超过 60%。[1]