Acetylcysteine (NAC) is an N-acetyl derivative of cysteine, a ROS inhibitor and mucolytic agent. Acetylcysteine induces apoptosis, can be used to reduce mucus thickness, and has anti-influenza viral activity.

方法：人膀胱癌细胞 T24 用 Acetylcysteine (5-50 mM) 处理 24 h，使用 MTT 方法检测细胞活力。
结果：Acetylcysteine 剂量依赖性抑制 T24 细胞活力，IC50 为 33.33 mM。[1]
方法：大鼠心肌细胞 H9c2 用 Acetylcysteine (2-4 mM) 处理 12-24 h，使用 Flow Cytometry 方法检测细胞凋亡情况。
结果：Acetylcysteine 剂量和时间依赖性诱导 H9c2 细胞凋亡。[2]

方法：为研究对小鼠凋亡性肝损伤的影响，将 Acetylcysteine (150 mg/kg) 单次腹腔注射给 CD-1 小鼠，30 min 后 使用 GalN/LPS 诱导肝损伤。
结果：Acetylcysteine 预处理显著减轻了 GalN/LPS 诱导的肝细胞凋亡。Acetylcysteine 通过其强大的 ROS 清除和抗凋亡作用减轻 GalN/LPS 诱导的凋亡性肝损伤。[3]
方法：为检测体内活性，将 Acetylcysteine (500 mg/kg) 口服给药给亨廷顿舞蹈症 (HD)的 R6/1 转基因小鼠，每天一次，持续九周。
结果：慢性 Acetylcysteine 给药延迟了 R6/1 小鼠运动缺陷的发生和发展，同时对 R6/1 和野生型小鼠都具有抗抑郁样作用。[4]

For survival experiments, washed cells are resuspended in RPM1 1640 medium and plated in 0.5 mL at a density of 8-10×105 per well in 24 well plastic culture dishes coated with rat tail collagen. To feed, but to avoid loss of floating cells, fresh medium (0.2 mL) is added to the cultures on days 1, 5, and 10. For experiments involving 'primed' PC12 cells, cultures are pretreated for l-2 weeks with NGF in RPM1 1640 medium supplemented with 1% heat-iN-acetylcysteinetivated horse serum. The cells are then washed and passaged into serum-free RPM1 1640 medium.

Rats are randomly allocated into five groups: sham group (n=5), control group with IIR (n=8) and three groups with IIR who are given N-acetylcysteine in different dosages: 150 mg/kg intraperitoneally 5 min before ischemia (n=8, group N-acetylcysteine 150), 300 mg/kg i.p 5 min before ischemia (n=7, group N-acetylcysteine 300), and 150 mg/kg i.p 5 min before ischemia plus 150 mg/kg 5 min before reperfusion (n=7, group N-acetylcysteine 150 + 150). After 4 h of reperfusion, the animals are euthanized by exsanguination from the abdominal aorta.[1]