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Influenza A H1N1 (A/California/04/2009) Hemagglutinin/HA-specific B cell probe (Y108F, His & Avi), Biotinylated

产品编号 TMPY-06927

Influenza A H1N1 (A/California/04/2009) Hemagglutinin/HA-specific B cell probe (Y108F, His & Avi), Biotinylated is expressed in Baculovirus insect cells with His and Avi tag. The predicted molecular weight is 59.58 kDa and the accession number is ACP41105.1.

Influenza A H1N1 (A/California/04/2009) Hemagglutinin/HA-specific B cell probe (Y108F, His & Avi), Biotinylated

Influenza A H1N1 (A/California/04/2009) Hemagglutinin/HA-specific B cell probe (Y108F, His & Avi), Biotinylated

产品编号 TMPY-06927
Influenza A H1N1 (A/California/04/2009) Hemagglutinin/HA-specific B cell probe (Y108F, His & Avi), Biotinylated is expressed in Baculovirus insect cells with His and Avi tag. The predicted molecular weight is 59.58 kDa and the accession number is ACP41105.1.
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20 μg¥ 3,1705日内发货
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产品信息

生物活性
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
Influenza A H1N1 (A/California/04/2009) Hemagglutinin/HA-specific B cell probe (Y108F, His & Avi), Biotinylated is expressed in Baculovirus insect cells with His and Avi tag. The predicted molecular weight is 59.58 kDa and the accession number is ACP41105.1.
种属
H1N1
表达系统
Baculovirus Insect Cells
标签C-His-Avi
蛋白编号ACP41105.1
别名
Harvey rat sarcoma viral oncogene homolog
蛋白构建
A DNA sequence encoding the Influenza A virus ((A/California/04/2009) (H1N1)) hemagglutinin (translated amino acids of ACP41105.1) (Y108F, Met1-Gly519), termed as HA-specific B cell probe, was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Asp 18
蛋白纯度
≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
分子量59.58 kDa (predicted); 66.9 kDa (reducing conditions)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 300 mM NaCl, 10% Glycerol, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.

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