Zika virus (ZIKV) (strain Zika SPH2016) ZIKV-E (Stem/anchor domain of flavivirus envelope glycoprotein E) protein (Fc) is expressed in HEK293 mammalian cells with Fc tag. The predicted molecular weight is 36.7 kDa and the accession number is ALU33341.1.

ZIKV

HEK293 Cells

A DNA sequence encoding the Zika virus (strain Zika SPH2016) E_stem (Stem/anchor domain of flavivirus envelope glycoprotein E) (ALU33341.1) (Gly698-Ala794) was expressed with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Gly 698

> 95 % as determined by SDS-PAGE.

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Envelope of Zika virus is responsible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent with those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross-reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross-reactivity with dengue and YF.Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus.