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Moloney murine leukemia virus (MoMLV) (isolate Shinnick) Gag polyprotein (GST)

产品编号 TMPH-02465

Moloney murine leukemia virus (MoMLV) (isolate Shinnick) Gag polyprotein (GST) is expressed in E. coli expression system with N-GST tag. The predicted molecular weight is 57.6 kDa and the accession number is P03332.

Moloney murine leukemia virus (MoMLV) (isolate Shinnick) Gag polyprotein (GST)

Moloney murine leukemia virus (MoMLV) (isolate Shinnick) Gag polyprotein (GST)

产品编号 TMPH-02465
Moloney murine leukemia virus (MoMLV) (isolate Shinnick) Gag polyprotein (GST) is expressed in E. coli expression system with N-GST tag. The predicted molecular weight is 57.6 kDa and the accession number is P03332.
规格价格库存数量
20 μg¥ 2,29020日内发货
100 μg¥ 4,32020日内发货
1 mg¥ 14,60020日内发货
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生物活性

生物活性
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
Moloney murine leukemia virus (MoMLV) (isolate Shinnick) Gag polyprotein (GST) is expressed in E. coli expression system with N-GST tag. The predicted molecular weight is 57.6 kDa and the accession number is P03332.
种属
MoMLV
表达系统
E. coli
标签N-GST
蛋白编号P03332
别名
gag,Gag polyprotein,Core polyprotein
氨基酸序列
PLRAGGNGQLQYWPFSSSDLYNWKNNNPSFSEDPGKLTALIESVLITHQPTWDDCQQLLGTLLTGEEKQRVLLEARKAVRGDDGRPTQLPNEVDAAFPLERPDWDYTTQAGRNHLVHYRQLLLAGLQNAGRSPTNLAKVKGITQGPNESPSAFLERLKEAYRRYTPYDPEDPGQETNVSMSFIWQSAPDIGRKLERLEDLKNKTLGDLVREAEKIFNKRETPEEREERIRRETEEKEERRRTEDEQKEKERDRRRHREMSKLL
蛋白构建
216-478 aa
蛋白纯度
> 90% as determined by SDS-PAGE.
分子量57.6 kDa (predicted)
缓冲液Tris-based buffer, 50% glycerol
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景
Plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably links the viral protein to the host ESCRT pathway and facilitates release.; Targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the pre-integration complex.; Constituent of the pre-integration complex (PIC) which tethers the latter to mitotic chromosomes.; Forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex.; Involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved UCUG elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization.

计算器

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