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IL-5R alpha/CD125 Protein, Human, Recombinant(aa 1-335, His)
产品编号 TMPY-01871
IL-5R alpha/CD125 Protein, Human, Recombinant(aa 1-335, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 37.3 kDa and the accession number is Q01344-2.
IL-5R alpha/CD125 Protein, Human, Recombinant(aa 1-335, His)
产品编号 TMPY-01871
IL-5R alpha/CD125 Protein, Human, Recombinant(aa 1-335, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 37.3 kDa and the accession number is Q01344-2.
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μg | ¥ 3,170 | 现货 | |
| 100 μg | ¥ 4,920 | 5日内发货 | |
| 200 μg | ¥ 7,620 | 5日内发货 | |
| 500 μg | ¥ 13,570 | 5日内发货 |
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产品信息
| 生物活性 | 1. Measured by its binding ability in a functional ELISA.
2. Immobilized recombinant mouse IL5-his at 5 μg/mL (100 μL/well) can bind biotinylated human IL5Ra-his with a linear range of 32-4000 ng/mL.
3. Measured by its ability to inhibit IL5 dependent proliferation of TF1 human erythroleukemic cells. The ED50 for this effect is typically 0.7-2.8 μg/mL.  |
| 产品描述 | IL-5R alpha/CD125 Protein, Human, Recombinant(aa 1-335, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 37.3 kDa and the accession number is Q01344-2. |
| 种属 | Human |
| 表达系统 | HEK293 Cells |
| 标签 | C-His |
| 蛋白编号 | Q01344-2 |
| 别名 | interleukin 5 receptor, α,interleukin 5 receptor, alpha,IL5R,HSIL5R3,CDw125,CD125 |
| 蛋白构建 | A DNA sequence encoding the human IL5Ra isoform 2 (NP_783852.1) (Met 1-Arg 335) was expressed, fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Asp 21 |
| 蛋白纯度 | > 85 % as determined by SDS-PAGE  |
| 分子量 | 37.3 kDa (predicted); 45-50 kDa (reducing condition, due to glycosylation) |
| 内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
| 缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| 复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| 存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. |
| 研究背景 | Interleukin 5 receptor, alpha (IL5RA) also known as CD125 (Cluster of Differentiation 125) is a subunit of the Interleukin-5 receptor. IL5RA (CD125) is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand-specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony-stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Six alternatively spliced transcript variants encoding three distinct isoforms have been reported. IL5RA (CD125) is a T-cell-derived cytokine that is particularly important in the development of asthma for the terminal differentiation, activation, and survival of committed eosinophil precursors. |
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