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Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease. IFN gamma Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 19.1 kDa and the accession number is P01579.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
100 μg | ¥ 5,180 | 5日内发货 | |
500 μg | ¥ 20,700 | 5日内发货 | |
1 mg | ¥ 34,600 | 5日内发货 |
生物活性 | Immobilized Human IFNGR1, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IFN gamma, His Tag with the EC50 of 39.1g/ml determined by ELISA. |
产品描述 | Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease. IFN gamma Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 19.1 kDa and the accession number is P01579. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-His-Avi |
蛋白编号 | P01579 |
别名 | IFNγ,IFN γ/IFNG,gamma,γ,IFG,IFN γ,IFN gamma,Interferon-gamma,interferon,IFI,Interferon-γ,干扰素 |
蛋白构建 | Gln24-Gly161 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE |
分子量 | 19.1 kDa (predicted). Due to glycosylation, the protein migrates to 25-40 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease. |
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