DDR2 Protein, Human, Recombinant (His & Avi), Biotinylated

产品编号 TMPY-07005

DDR2 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is Q16832.

产品编号 TMPY-07005

DDR2 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is Q16832.

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产品信息

生物活性	Loaded DDR2 Protein, Human, Recombinant (His & Avi), Biotinylated (Cat#TMPY-07005) on SA Biosensor, can bind Native Human Collagen I with an affinity constant of 1.37 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
产品描述	DDR2 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is Q16832.
种属	Human
表达系统	HEK293 Cells
标签	C-His-Avi
蛋白编号	Q16832
蛋白构建	A DNA sequence encoding the Human DDR2 (NP_001014796.1) (Met1-Arg399) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
蛋白纯度	≥ 95% as determined by SDS-PAGE. ≥ 95% as determined by SEC-HPLC.
分子量	45.84 kDa (predicted); 60.4 kDa (reducing contition)
内毒素	< 1.0 EU per μg protein as determined by the LAL method.
缓冲液	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
复溶方法	Please refer to the lot-specific COA.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Discoidin domain receptor 2 (DDR2) or CD167b (cluster of differentiation 167b) is a kind of protein tyrosine kinases associated with cell proliferation and tumor metastasis, and collagen, identified as a ligand for DDR2, up-regulates matrix metallloproteinase 1 (MMP-1) and MMP-2 expression in cellular matrix. DDR2/CD167b was found to recognise the triple-helical region of collagen X as well as the NC1 domain. Binding to the collagenous region was dependent on the triple-helical conformation. DDR2/CD167b autophosphorylation was induced by the collagen X triple-helical region but not the NC1 domain, indicating that the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation. DDR2/CD167b is induced during stellate cell activation and implicate the phosphorylated receptor as a mediator of MMP-2 release and growth stimulation in response to type I collagen. Moreover, type I collagen-dependent upregulation of DDR2/CD167b expression establishes a positive feedback loop in activated stellate cells, leading to further proliferation and enhanced invasive activity.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

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