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CD40 Ligand Protein, Mouse, Recombinant (monomeric hFc)
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很棒货号 TMPK-00860
别名 TRAP, TNFSF5IMD3, TNFSF5, T-BAM, IMD3, IGM, HIGM1, gp39, CD40LIGM, CD40LG, CD40L, CD40 Ligand, CD154
CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells. CD40 Ligand Protein, Mouse, Recombinant (monomeric hFc) is expressed in HEK293 mammalian cells with N-monomeric hFc tag. The predicted molecular weight is 75.6 kDa and the accession number is P27548.
其他形式的 “CD40 Ligand Protein, Mouse, Recombinant (monomeric hFc)”:
CD40 Ligand Protein, Mouse, Recombinant (monomeric hFc)
一键复制产品信息 很棒货号 TMPK-00860 别名 TRAP, TNFSF5IMD3, TNFSF5, T-BAM, IMD3, IGM, HIGM1, gp39, CD40LIGM, CD40LG, CD40L, CD40 Ligand, CD154
CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells. CD40 Ligand Protein, Mouse, Recombinant (monomeric hFc) is expressed in HEK293 mammalian cells with N-monomeric hFc tag. The predicted molecular weight is 75.6 kDa and the accession number is P27548.
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 5 μg | ¥ 298 | 现货 | |
| 10 μg | ¥ 472 | 现货 | |
| 20 μg | ¥ 768 | 3日内发货 | |
| 50 μg | ¥ 1,490 | 3日内发货 | |
| 100 μg | ¥ 2,510 | 现货 | |
| 200 μg | ¥ 4,530 | 5日内发货 | |
| 500 μg | ¥ 10,000 | 5日内发货 |
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纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
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| 产品描述 | CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells. CD40 Ligand Protein, Mouse, Recombinant (monomeric hFc) is expressed in HEK293 mammalian cells with N-monomeric hFc tag. The predicted molecular weight is 75.6 kDa and the accession number is P27548. |
| 生物活性 | Immobilized Mouse CD40, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 25.6ng/ml determined by ELISA. |
| 研究背景 | CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells. |
| 种属 | Mouse |
| 表达系统 | HEK293 Cells |
| 标签 | N-monomeric hFc |
| 蛋白编号 | P27548 |
| 蛋白构建 | Met112-Leu260 |
| 蛋白纯度 | > 95% as determined by Tris-Bis PAGE |
| 蛋白性状 | Lyophilized powder |
| 缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, 200 mM L-arginine (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| 复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| 别名 | TRAP, TNFSF5IMD3, TNFSF5, T-BAM, IMD3, IGM, HIGM1, gp39, CD40LIGM, CD40LG, CD40L, CD40 Ligand, CD154 |
| 内毒素 | < 1 EU/μg by the LAL method. |
| 分子量 | 75.6 kDa (predicted). Due to glycosylation, the protein migrates to 76-80 kDa based on Tris-Bis PAGE result. |
| 颜色 | White |
| 物理性状 | Lyophilized powder |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. |
| 存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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