VEGFR-2/DHFR-IN-1 (compound 8b) is a chemical inhibitor targeting VEGFR-2 and DHFR, exhibiting IC50 values of 0.384 μM and 7.881 μM, respectively. It demonstrates effective antibacterial properties against a range of pathogens including Escherichia coli, Streptococcus faecalis, Salmonella enterica, MSSA, and MRSA, with MIC values ranging from 8 to 16 μg/mL. Additionally, this compound shows potent cytotoxic effects against various cancer cell lines such as C26, HepG2, and MCF7, with IC50 values between 2.97 and 7.12 μM, rendering it a valuable tool for cancer research [1].

VEGFR-2/DHFR-IN-1 (2-1024 μg/mL; 18-72 h) shows antibacterial and antifungal activities with MIC values of 16, 64, 16, 16, 8, 16, 64 and 256 μg/mL for Escherichia coli, Pseudomonas aeruginosa, Streptococcus faecalis, Salmonella enterica, MSSA, MRSA, Candida albicans and Aspergillus niger, respectively [1]. VEGFR-2/DHFR-IN-1 (0.5-100 μM; 48 h) exhibits anticancer activities [1]. VEGFR-2/DHFR-IN-1 (0-100 μM; 30 min) shows DHFR and VEGFR-2 inhibitory activities with IC 50 s of 7.881 and 0.384 μM, respectively [1]. Cell Cytotoxicity Assay [1] Cell Line: C26, HepG2, MCF7 and H69PR cell lines Concentration: 0.5, 1, 5, 10, 25, 50, 80 and 100 μM Incubation Time: 48 hours Result: Showed anticancer activities to C26, HepG2, MCF7 and H69PR cells with IC 50 s of 2.97, 7.12, 3.58 and 12.79 μM, respectively.