Di-8-ANEPPS is a voltage-sensitive dye and fast-response probe that fluoresces in response to potential changes in the environment and is capable of detecting transient potential changes in cells, with a 2-10% fluorescence change per 100 mV and potential-dependent changes in the excitation spectrum.

在小鼠室间隔心肌细胞的膜电位实验中，Di-8-ANEPPS 被用作电压敏感染料，用于记录动作电位传播。使用浓度为 5 μM，在心肌组织灌流 20 分钟后开始记录电信号。Di-8-ANEPPS 不影响动作电位波形，但抑制了 Connexin43 缝隙连接蛋白的磷酸化水平，从而降低了缝隙连接通量（gap junctional conductance）和动作电位的传导速度[1]。