Chrysoidine G (free base) (Solvent Orange 3; Chrysoidine Y base) serves as a versatile dye utilized across various domains. Primarily, dyes are essential in biological experiments for observing and analyzing cell structures, tracking biomolecules, evaluating cell functions, distinguishing cell types, detecting biomolecules, investigating tissue pathology, and monitoring microorganisms. Beyond basic scientific research and clinical diagnostics, dyes like Chrysoidine G find applications in traditional industries such as textile dyeing and emerging sectors including functional textile processing, food pigments, and dye-sensitized solar cells.