Acyl coenzyme A synthetase (ACS), also known as acetyl coenzyme A synthetase, plays a pivotal role in biochemical research. This enzyme catalyzes the activation of fatty acids via coenzyme A through a two-step thioesterification process, resulting in the formation of acyl coenzyme A. Subsequently, it is involved in numerous anabolic and catabolic lipid metabolism pathways and contributes to the tricarboxylic acid (TCA) cycle during aerobic respiration [1].