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Nectin-4 Protein, Human, Recombinant (hFc & Avi), Biotinylated

产品编号 TMPY-06958
Nectin-4 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with hFc and Avi tag. The predicted molecular weight is 62.65 kDa and the accession number is NP_112178.2.
规格价格库存数量
20 μg¥ 3,8205日内发货
100 μg¥ 8,8405日内发货
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生物活性

生物活性
Immobilized Recombinant Human NECTIN4/Nectin 4 Protein (ECD, Fc & AVI Tag), Biotinylated on streptavidin precoated (2 μg/mL,100 μL/well) can bind Recombinant Human CD111 / Nectin-1 / PVRL1 Protein (His Tag), the EC50 is 40-120 ng/mL.
产品描述
Nectin-4 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with hFc and Avi tag. The predicted molecular weight is 62.65 kDa and the accession number is NP_112178.2.
种属
Human
表达系统
HEK293 Cells
标签C-hFc-Avi
蛋白编号NP_112178.2
别名
LNIR,PVRL4,EDSS1,PRR4,nectin-4
蛋白构建
A DNA sequence encoding the Human NECTIN4 (NP_112178.2) (Met1-Ser349) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Gly 32
蛋白纯度
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
分子量62.65 kDa (predicted); 76.3 kDa (reducing conditions)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.

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