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Nectin-4 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with hFc and Avi tag. The predicted molecular weight is 62.65 kDa and the accession number is NP_112178.2.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
20 μg | ¥ 3,820 | 5日内发货 | |
100 μg | ¥ 8,840 | 5日内发货 |
生物活性 | Immobilized Recombinant Human NECTIN4/Nectin 4 Protein (ECD, Fc & AVI Tag), Biotinylated on streptavidin precoated (2 μg/mL,100 μL/well) can bind Recombinant Human CD111 / Nectin-1 / PVRL1 Protein (His Tag), the EC50 is 40-120 ng/mL. |
产品描述 | Nectin-4 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with hFc and Avi tag. The predicted molecular weight is 62.65 kDa and the accession number is NP_112178.2. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-hFc-Avi |
蛋白编号 | NP_112178.2 |
别名 | PVRL4,PRR4,nectin-4,LNIR,EDSS1 |
蛋白构建 | A DNA sequence encoding the Human NECTIN4 (NP_112178.2) (Met1-Ser349) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Gly 32 |
蛋白纯度 | ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. |
分子量 | 62.65 kDa (predicted); 76.3 kDa (reducing conditions) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. |
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