B3GNT2 belongs to the beta-1,3-N-acetylglucosaminyltransferase family. It is a type II transmembrane protein that prefers the substrate of lacto-N-neotetraose. Alternative splicing produced 2 isoforms of the human protein. B3GNT2 catalyzes the initiation and elongation of poly-N- acetyllactosamine chains. Enzymatic activities of some glycosyltransferases are markedly increased via complex formation with other transferases or cofactor proteins. B3GNT2 and beta3Gn-T8 can form a heterodimer in vitro and that the complex exhibits much higher enzymatic activity than either enzyme alone. It is found that up-regulation of beta3Gn-T8 in differentiated HL-60 cells may increase poly-N-acetyllactosamine chains by activating intrinsic B3GNT2.
生物活性 | Testing in progress |
产品描述 | B3GNT2 belongs to the beta-1,3-N-acetylglucosaminyltransferase family. It is a type II transmembrane protein that prefers the substrate of lacto-N-neotetraose. Alternative splicing produced 2 isoforms of the human protein. B3GNT2 catalyzes the initiation and elongation of poly-N- acetyllactosamine chains. Enzymatic activities of some glycosyltransferases are markedly increased via complex formation with other transferases or cofactor proteins. B3GNT2 and beta3Gn-T8 can form a heterodimer in vitro and that the complex exhibits much higher enzymatic activity than either enzyme alone. It is found that up-regulation of beta3Gn-T8 in differentiated HL-60 cells may increase poly-N-acetyllactosamine chains by activating intrinsic B3GNT2. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | N-hFc |
蛋白编号 | Q9NY97-1 |
别名 | B3GNT-2, B3GNT, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2, UDP-GlcNAc:βGal β-1,3-N-acetylglucosaminyltransferase 2, β3GNT, BGNT2, BETA3GNT, BGnT-2, B3GN-T2, B3GNT1 |
蛋白构建 | The Human B3GNT2 (Q9NY97-1) (Lys29-Cys397) was expressed,with the fused Fc region of Human IgG1 at the N-terminus. |
蛋白纯度 | > 90 % as determined by SDS-PAGE |
分子量 | 71.2 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | B3GNT2 belongs to the beta-1,3-N-acetylglucosaminyltransferase family. It is a type II transmembrane protein that prefers the substrate of lacto-N-neotetraose. Alternative splicing produced 2 isoforms of the human protein. B3GNT2 catalyzes the initiation and elongation of poly-N- acetyllactosamine chains. Enzymatic activities of some glycosyltransferases are markedly increased via complex formation with other transferases or cofactor proteins. B3GNT2 and beta3Gn-T8 can form a heterodimer in vitro and that the complex exhibits much higher enzymatic activity than either enzyme alone. It is found that up-regulation of beta3Gn-T8 in differentiated HL-60 cells may increase poly-N-acetyllactosamine chains by activating intrinsic B3GNT2. |
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B3GNT2 Protein, Human, Recombinant (hFc) B3GNT-2 B3GNT UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 UDP-GlcNAc:βGal β-1,3-N-acetylglucosaminyltransferase 2 β3GNT BGNT2 BETA3GNT BGNT-2 BGnT-2 B3GN-T2 BGNT 2 B3GNT1 recombinant recombinant-proteins proteins protein