SCH28080 is a reversible and K+-competitive inhibitor of gastric H+/K+-ATPase with an IC50 value of 20 nM (rabbit microsomal membrane).SCH28080 is a potent inhibitor of acid secretion in vivo with anti-ulcer activity, anti-secretory and cytoprotective activity.

H+, K+-ATPase, rabbit microsomal membranes:20 nM, H+, K+-ATPase:0.12 μM(Ki), Aminopyrine:0.029 μM, INS-1E cells:22.9 µM, INS-1E cells:15.3 µM

SCH28080 competitively hinders ATP hydrolysis stimulated by K+, with a Ki value of 0.12 μM.[1]
SCH28080 effectively suppresses histamine-induced uptake of [14C]aminopyrine in isolated rabbit parietal cells, demonstrating an IC50 of 0.029 μM.[1]
SCH28080 elicits a dose-dependent decrease in cell viability, with IC50 values of 22.9 µM and 15.3 µM following 2-hour and 24-hour treatments, respectively. And at concentration of 100 µM, cell viability drops below 10% as early as 2 hours.[2]
SCH28080 induces apoptosis and exhibits cytotoxicity at higher doses.[2].
SCH28080 also impedes insulin secretion by activating IK ATP and inhibiting L-type voltage-gated Ca2+ channels, ultimately reducing cell viability and induces apoptosis/necrosis in a dose-dependent manner.[2]

SCH28080 (20 mg/kg; i.p.) effectively attenuates gastric ulcers induced by pylorus ligation in rats.[3]